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Hat three clones ZK1, ZK2 and ZK6 were obtained by limiting dilution and further characterized. Our PCR genotyping results verified these three clones, ZK1, ZK2 and ZK6 are MARCO-/- and SR-AI/ II-/--deficient (Fig. 1). These cell lines are able to grow rapidly in RPMI or DMEM complete media in the absence of exogenous M-CSF or other growth factors, and their doubling time is 12?6 h on the average

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